Cre-loxP, a powerful tool for preclinical cancer research, possesses intrinsic pitfalls that can alter result accuracy, according to an editorial paper in Oncoscience. Cancer and other diseases could ...

A new editorial paper was published in Oncoscience (Volume 10) on November 14, 2023, entitled, "Be mindful of potential pitfalls when using the Cre-LoxP system in cancer research." In this new ...

Researchers at the University of Tsukuba have developed a vector that integrates the Cre enzyme gene with the specific DNA ...

Here we describe the generation of a double-fluorescent Cre/loxP indicator system. This protocol involves (i) all cloning steps to generate the plasmid vector (3–5 months); (ii) a guide to prepare ...

To generate a tissue specific knockout mouse, researchers breed a mouse bearing a Cre transgene under a tissue- or cell-type specific or inducible promoter (A) with a homozygous floxed mouse (B). The ...

Chances are if you use mice in your research, you are intimately acquainted with the Cre/LoxP recombination system. (For the uninitiated out there, this is a system to achieve tissue- or cell ...

“One limitation [of Cre-LoxP], the focus of this editorial, is the potential loss of fidelity of Cre recombinase expression especially in the context of modeling cancer in mice.” “It is based on ...

Mice and newts were applied to the one-step Cre-loxP organism creation technology by TAx9. In this study, the researchers succeeded in synthesizing a Cre-loxP integrated vector by placing a short DNA ...