CRISPR-Cas9 has been widely adapted for use in transcriptional modulation and epigenetic engineering with deactivated Cas9 (dCas9) systems to enable CRISPR interference (CRISPRi) and CRISPR activation ...
Advancements in transgenic technology since 1983 have led to the widespread use of the 35S promoter, which lacks specificity and can be lethal. To overcome these limitations, research has shifted to ...
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