Because of its reliability, sensitivity and low cost, PCR has become the backbone of modern diagnostics. In recent years, researchers have ingeniously capitalized on the fundamental nature of PCR ...

Designing reproducible PCR assays involves optimizing multiple moving targets, from standardizing each component in sometimes minute reaction volumes to planning ahead to ensure long-term and secure ...

The key to an optimal PCR experiment is primer design. In general, primers should be between 18 and 24 nucleotides long and have a GC content between 40 and 60 percent. Ideally primers should contain ...