Stick a soda straw into a glass of water, and from the side, the straw will appear to bend at the interface between air and water. That effect is caused by the different refractive indexes of the air ...

In TIRF microscopy, fluorescent molecules are in a sample in an aqueous environment that is near a solid with a high refractive index, usually a glass coverslip. At what is called the critical angle, ...

Recent technological advances in conjunction with major developments in fluorescent markers have made fluorescence microscopy an extremely powerful tool. René Hessling and Thorsten Kues of Carl Zeiss ...

TIRF is a microscopy technique that is used to image fluorescent molecules, such as green fluorescent protein (GFP) and fluorochromes, in liquids that are adjacent to a solid with a high refractive ...

The DeltaVision OMX V4 enables Total Internal Reflection Fluorescence Microscopy (TIRF). This technique uses an evanescent wave to selectively excite fluorophores that are close to the coverslip. This ...