We see two new Na'vi tribes in the trailer: the Wind Traders and the Ash People clan, the latter of whom seem to represent most of the menace in the film. We see the Ash People using fire as a weapon, with one of the aliens even seeming to be able to wield it on her bare hands without hurting herself.

At CinemaCon 2025 in April, Oscar-winner Saldaña added that Fire and Ash will introduce a second new clan called the wind traders, whom she described as " a peaceful, nomadic air-traveling clan ," as well as the Ash People, whom Saldaña said have "forsaken" the Na'vi god.